We then examined the effect of a small molecule inhibitor of Bag 1 Knockoff Hermes Bag, Thio 2, in Bag 1L driven premalignant change in this experimental model of ductal carcinoma in situ to determine whether these changes might be amenable to therapeutic intervention.Top of pageResultsCharacterization of Bag 1L overexpression in 2D cultureTo examine the potential role of Bag 1L in breast tumourigenesis Replica Hermes Birkin, MCF 10A stable cell clones were generated by transfection of a pcDNA3 vector containing Bag 1 complementary DNA with an optimized Bag 1L start site.22 Immunoblot analysis revealed the presence of two clones overexpressing Bag 1L at low (Bag 1L and high (Bag 1L levels compared with two clones containing empty pcDNA3 vector of which clone 1, designated pcDNA Replica Hermes Birkin, was used as a control for this study (Figure 1a). Immunofluorescence staining revealed higher but heterogeneous expression of Bag 1 in the nucleus of both clones compared with pcDNA and was more intense in Bag 1L (Figure 1b), which is consistent with overexpression of Bag 1L. Values represent the mean from three independent experiments, each with three technical replicates.
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